Journal: Human Vaccines & Immunotherapeutics
Article Title: Mucosal immunization with an adenoviral vector expressing a prefusogenic F protein protects the upper and lower respiratory tracts of cotton rats against live respiratory syncytial virus challenge
doi: 10.1080/21645515.2026.2623573
Figure Lengend Snippet: ChAd68-PreF elicits robust anti-RSV F binding IgG responses in cotton rats. (A), immunization and challenge schedule. Animals received a homologous prime-boost (day 0 and 28) and were intranasally challenged with 1 × 10 5 PFU RSV A2 on day 56, except the unchallenged ChAd68-GFP/UnCh group. (B), Serum binding IgG antibody titers (ELISA) at days 0, 28, 56 and 61. The symbols denote individual animals; bars show mean ± SD. Statistics: one-way ANOVA with Tukey’s multiple-comparisons test (* p < .05; ** p < .01; *** p < .001; **** p < .0001).
Article Snippet: Neutralization assays in cotton rats were performed using the following strains: RSV A2 (ATCC VR-1540), RSV B (strain 18537; ATCC VR-1580PQ), and RSV Long (ATCC VR-26).
Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay